{"id":869,"date":"2011-05-17T09:58:14","date_gmt":"2011-05-17T07:58:14","guid":{"rendered":"http:\/\/slanedeti.sk\/cms\/sekvencovanie-dna-super-bakterie-u-pacientov-s-cystickou-fibrozou-2\/201105"},"modified":"2014-02-09T15:49:02","modified_gmt":"2014-02-09T14:49:02","slug":"sekvencovanie-dna-super-bakterie-u-pacientov-s-cystickou-fibrozou-2","status":"publish","type":"post","link":"https:\/\/slanedeti.sk\/cms\/sekvencovanie-dna-super-bakterie-u-pacientov-s-cystickou-fibrozou-2\/869","title":{"rendered":"Sekvencovanie DNA \u201esuper bakt\u00e9rie\u201c u pacientov s cystickou fibr\u00f3zou"},"content":{"rendered":"

Bakt\u00e9ria s n\u00e1zvom Pseudomonas aeruginosa je naj\u010dastej\u0161ou pr\u00ed\u010dinou persistentn\u00fdch a fat\u00e1lnych infekci\u00ed u pacientov s cystickou fibr\u00f3zou. Vedci v Liverpoole identifikovali zvl\u00e1\u0161\u0165 virulentn\u00fd kme\u0148 bakt\u00e9ri\u00ed, prenosn\u00fdch medzi pacientov. Liverpoolsky\u00a0\u00a0 epidemick\u00fd kme\u0148 (LES), ozna\u010dovan\u00fd ako \u201esuperbug\u201c (\u201esuper bakt\u00e9ria\u201c, odoln\u00e1 vo\u010di antibiotik\u00e1m), m\u00f4\u017ee sp\u00f4sobi\u0165 agres\u00edvnu infekciu a vies\u0165 k progres\u00edvnemu oslabeniu p\u013e\u00facnych funkci\u00ed.<\/p>\n

V\u00fdskumn\u00fd t\u00edm z In\u0161tit\u00fatu infek\u010dn\u00fdch chor\u00f4b a celosvetov\u00e9ho zdravia Liverpoolskej univerzity odobral pacientom vzorky zo sp\u00fata a\u00a0 v\u00fdterov, aby pochopil, pre\u010do je infekcia u \u013eud\u00ed s cystickou fibr\u00f3zou tak\u00e1 agres\u00edvna. Zistilo sa, \u017ee v priebehu chronick\u00fdch infekci\u00ed tieto bakt\u00e9rie dok\u00e1\u017eu r\u00fdchlo mutova\u0165, \u010do m\u00e1 za n\u00e1sledok obrovsk\u00fa r\u00f4znorodos\u0165. Testy tie\u017e uk\u00e1zali, \u017ee bakt\u00e9rie produkuj\u00fa molekulu, ktor\u00e1 by mohla by\u0165 sp\u00fa\u0161\u0165a\u010dom z\u00e1chvatov ak\u00fatnej infekcie u pacientov.<\/p>\n

Dr. Craig Winstanley, \u010dlen Biomedic\u00ednskeho v\u00fdskumn\u00e9ho centra (BRC) N\u00e1rodn\u00e9ho \u00fastavu pre medic\u00ednsky v\u00fdskum (NIHR) v Liverpoole, vysvet\u013euje: \u201e Pacienti s LES musia by\u0165 v nemocniciach oddelen\u00ed od ostatn\u00fdch pacientov, aby sa na oddeleniach infekcia ne\u0161\u00edrila medzi pacientmi s cystickou fibr\u00f3zou.\u00a0 Ako n\u00e1hle tieto infekcie vznikn\u00fa, nikdy sa ich u\u017e nemo\u017eno zbavi\u0165. Zistili sme, \u017ee tieto bakt\u00e9rie maj\u00fa schopnos\u0165 diverzifik\u00e1cie do stoviek rozdielnych podtypov, v d\u00f4sledku \u010doho je ve\u013emi \u0165a\u017ek\u00e9 rozhodn\u00fa\u0165, ktor\u00e9 antibiotikum by sa malo pou\u017ei\u0165 , aby v\u00fdsledok bol \u00faspe\u0161n\u00fd.<\/p>\n

\u201ePomocou najnov\u0161ej technol\u00f3gie sekvencovania DNA m\u00e1me jedine\u010dn\u00fa pr\u00edle\u017eitos\u0165 sk\u00fama\u0165 spr\u00e1vanie bakt\u00e9ri\u00ed v priebehu chronickej infekcie v re\u00e1lnom \u010dase. To n\u00e1m umo\u017en\u00ed z\u00edska\u0165 jasnej\u0161\u00ed obraz o tom, ako sa bakt\u00e9ria tak efekt\u00edvne adaptuje na pacientov s cystickou fibr\u00f3zou. Ak pochop\u00edme, ako a pre\u010do sa bakt\u00e9ria spr\u00e1va ur\u010dit\u00fdm sp\u00f4sobom, budeme schopn\u00ed\u00a0 vybra\u0165 efekt\u00edvnej\u0161ie postupy lie\u010dby tejto infekcie\u201c.<\/p>\n

Pri v\u00fdskumnej pr\u00e1ci s vedeck\u00fdmi pracovn\u00edkmi\u00a0 v Centre pre v\u00fdskum gen\u00f3mu (CGR) Liverpoolskej univerzity, t\u00edm pou\u017eije nov\u00fa technol\u00f3giu sekvencovania DNA na pre\u010d\u00edtanie genetick\u00e9ho k\u00f3du infekcie. Ako prv\u00fd svojho druhu vo Ve\u013ekej Brit\u00e1nii pracuje stroj 250 000 razy r\u00fdchlej\u0161ie ako technol\u00f3gia pou\u017eit\u00e1 na sekvencovanie \u013eudsk\u00e9ho gen\u00f3mu pred 10 rokmi.<\/p>\n

Dr. Steve Paterson z In\u0161tit\u00fatu integrat\u00edvnej biol\u00f3gie Liverpoolskej univerzity, povedal: \u201eKa\u017ed\u00fd pacient s cystickou fibr\u00f3zou m\u00f4\u017ee by\u0165 infikovan\u00fd odli\u0161nou popul\u00e1ciou bakt\u00e9ri\u00ed a preto je k\u013e\u00fa\u010dov\u00e9 testova\u0165 vzorky choroby od viacer\u00fdch pacientov, aby sme pochopili, ako sa vyv\u00edja. Technol\u00f3gia, ktor\u00fa pou\u017e\u00edvame, je schopn\u00e1 pre\u010d\u00edta\u0165 30 bili\u00f3nov znakov re\u0165azca DNA denne, v porovnan\u00ed so \u0161tyrmi bili\u00f3nmi, ktor\u00e9 pre\u010d\u00edtame s pomocou dne\u0161n\u00fdch strojov. To n\u00e1m umo\u017en\u00ed\u00a0 ve\u013emi presne presk\u00fama\u0165 mut\u00e1cie tejto infekcie a z\u00edska\u0165 tak cenn\u00e9 inform\u00e1cie o v\u00fdvoji tohto v\u00e1\u017eneho zdravotn\u00e9ho stavu.\u201c<\/p>\n

Pozn\u00e1mky:<\/em>
\n V\u00fdskum financuje Wellcome Trust.<\/em>
\n Tento v\u00fdskum nadv\u00e4zuje na univerzitn\u00fa \u0161t\u00fadiu o Pseudomonas aeruginosa, publikovan\u00fa v \u010dasopise American Journal of Respiratory and Critical Care Medicine. V\u00fdskum, vykonan\u00fd v spolupr\u00e1ci s Dr. Martinom Walshawom z Liverpool Heart and Chest Hospital, podporili Dr. Hadwen Trust a NIHR prostredn\u00edctvom Liverpool Biomedical Research Centre.<\/em><\/p>\n

Zdroj:<\/em>
\n Samantha Martin University of Liverpool<\/em>
\n http:\/\/www.medicalnewstoday.com\/articles\/217182.php<\/a><\/em><\/p>\n","protected":false},"excerpt":{"rendered":"

Baktéria s názvom Pseudomonas aeruginosa je najčastejšou príčinou persistentných a fatálnych infekcií u pacientov s cystickou fibrózou. Vedci v Liverpoole identifikovali zvlášť virulentný kmeň baktérií, prenosných medzi pacientov. Liverpoolsky   epidemický kmeň (LES), označovaný ako „superbug“ („super baktéria“, odolná voči antibiotikám), … Continued<\/a><\/p>\n","protected":false},"author":3,"featured_media":1600,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"kt_blocks_editor_width":"","footnotes":""},"categories":[3],"tags":[158],"class_list":["post-869","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-lieky-a-liecba","tag-pseudomonas-aeruginosa"],"yoast_head":"\nSekvencovanie DNA \u201esuper bakt\u00e9rie\u201c u pacientov s cystickou fibr\u00f3zou - Slan\u00e9 deti<\/title>\n<meta name=\"description\" content=\"Naj\u010dastej\u0161ie sa vyskutuj\u00faca bakt\u00e9ria pri cystickej fibr\u00f3ze je Pseudomonas aeruginosa. 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